Did the Mouse Die? (Are you pregnant?)

Aschheim and Zondek discovered in 1927 that the urine of a pregnant woman would stimulate ovary growth in a mouse injected with this urine. This became the basis of the first biological pregnancy tests. The mouse did not die from the urine, but unfortunately had to be sacrificed to determine the test outcome, thus the saying, “Did the mouse die?” was coined.

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